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Aislamiento e identificación de bacterias ácido lácticas con potencial probiótico en bovinos Holstein. Colegio de Postgraduados
Caballero Cervantes, Yesenia.
El principal problema de salud en becerras de reemplazo son las diarreas provocadas por Escherichia coli enterotóxica. El uso de microorganismos pertenecientes al grupo de bacterias ácido lácticas (BAL) en la etapa de lactancia es benéfico para combatir la incidencia de diarreas e incrementar la ganancia de peso. Sin embargo, se ha reportado inconsistencia en los efectos benéficos de los productos comerciales. Por lo anterior, el objetivo de este estudio fue aislar y seleccionar BAL con potencial probiótico de mucosa oral de becerros lactantes, calostro y leche de vacas Holstein que puedan ser consideradas para el suministro de becerras en México. Para ello, se realizó el aislamiento en caldo Man Rogosa Sharp (MRS) y en agar MRS para la obtención de...
Palavras-chave: Becerras; Lactobacillus; Cepas; Resistencia; Heifers; Strains; Resistance; Ganadería; Maestría.
Ano: 2014 URL: http://hdl.handle.net/10521/2358
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Análise genética de três gerações de tilápia do Nilo (linhagem GIFT) utilizando o marcador RAPD - doi: 10.4025/actascianimsci.v33i2.9202 Animal Sciences
Oliveira, Sheila Nogueira; Universidade Estadual de Maringa; Ribeiro, Ricardo Pereira; Universidade Estadual de Maringa; Lopera, Nelson Mauricio; Universidade Estadual de Maringa; Candioto, Fernanda Braz; Universidade Estadual de Maringa; Resende, Emiko Kawakami; EMBRAPA; Legat, Angela Puchinik; EMBRAPA.
O objetivo deste trabalho foi analisar a diversidade genética de três gerações da tilápia GIFT do Programa de Melhoramento Genético da Universidade Estadual de Maringá, utilizando o marcador RAPD. Foram utilizadas 180 amostras de nadadeira. Os sete primers utilizados produziram 91 fragmentos, dos quais 98,9% foram polimórficos. Observaram-se diferenças (p < 0,05) na frequência de 64 fragmentos, dos quais dez foram excluídos na G2. Os resultados de variabilidade genética estimados pelo índice de Shannon (G0: 0,430; G1: 0,469 e G2: 0,420) e pela porcentagem de fragmentos polimórficos (G0: 90,1%; G1: 94,5% e G2: 86,8%) indicaram alta variabilidade genética intrapopulacional. Resultados esses que corroborando com os preceitos de diversidade genética de Nei...
Tipo: Analise laboratorial Palavras-chave: Produção Animal diversidade genética; Genetic Improvement of Farmed Tilapias; Linhagens; Melhoramento genético; Oreochromis niloticus Piscicultura genetic diversity; Genetic Improvement of Farmed Tilapias; Strains; Genetic improvement; Oreochromis niloticus.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/9202
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Comparison of the Quality Adjusting of Nonlinear Models for Organs, Carcass and Body Components in Meat-Type (Coturnix Coturnix Coturnix) and Laying-Type (Coturnix Coturnix Japonica) Quail Rev. Bras. Ciênc. Avic.
Grieser,DO; Furlan,AC; Ribeiro,PM; Zancanela,V; Del Vesco,AP; Gasparino,E; Zardin,AMSO; Marcato,SM.
ABSTRACT The objective of this study was to evaluate the adjustment quality of nonlinear models to data organs growth, carcass and body components of meat-type (Coturnix coturnix coturnix) and japanese laying-type (Coturnix coturnix japonica) quail. A total of 1350 quails from one to 42 d old were distributed in a completely randomized design, with five replicates each. To determine the organs growth (gut, heart, liver and gizzard), carcass and body components (wing, thigh and drumstick, back and breast), two quails per repetition were slaughtered weekly. The data were evaluated in function of different nonlinear models (Logistical, Brody, Richards, Von Bertalanffy and Gompertz). All models studied adjusted the data, differing in adjustment quality. Brody...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gompertz equation; Growth parameters; Breast; Number of interaction; Strains.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400701
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Crescimento micelial in vitro de cinco linhagens de Agaricus bisporus submetidas a diferentes condições de temperatura - DOI: 10.4025/actasciagron.v32i1.333 Agronomy
Andrade, Meire Cristina Nogueira de; INPA; Chavari, João Lucas; UNESP; Minhoni, Marli Teixeira de Almeida; UNESP; Zied, Diego Cunha; UNESP.
Avaliou-se o crescimento micelial in vitro das linhagens ABI-05/03, ABI-06/04, ABI-04/02, ABI-06/05 e ABI-01/01 de Agaricus bisporus em meios de cultura sólidos à base de composto. As avaliações foram realizadas por meio de medições de quatro diâmetros das colônias, a cada 48h, durante 12 dias de incubação, no escuro, a 20 e 25&ordm;C. O delineamento experimental foi o de blocos casualizados, com uso do teste de Tukey para a comparação das médias. Com base nos resultados obtidos, verificou-se que: o crescimento micelial de A. bisporus é influenciado pela temperatura de incubação; a temperatura de 25&ordm;C foi mais favorável para o crescimento micelial de todas as linhagens de A. bisporus; na temperatura de 20&ordm;C, o melhor crescimento foi...
Palavras-chave: 5.01.00.00-9 Agronomia champignon; Micélio; Cogumelo; Crescimento micelial; Linhagens champignon; Mycelium; Mushroom; Mycelium growth; Strains.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/333
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Digital monitoring of mycelium growth kinetics and vigor of shiitake (Lentinula edodes (Berk.) Pegler) on agar medium BJM
Montini,Renato Mamede de Castro; Passos,José Raimundo de Souza; Eira,Augusto Ferreira da.
The mycelium growth kinetics and vigor of shiitake (Lentinula edodes (Berk.) Pegler) strains LE 96/17, LE 98/51, LE 98/53, and LE 98/56 were studied under different agar medium compositions. The strains were from the mycological collection of the Módulo de Cogumelos, Faculdade de Ciências Agronômicas, Unesp-Botucatu, Brazil. Mycelium fragments from stock cultures were transferred to Petri dishes with Sawdust extract-Dextrose-Agar medium. The area of growth and vigor (density) of the mycelia were daily recorded with a digital camera, during incubation, until the complete colonization of the Petri dish. The images were analyzed by the freeware UTHSCSA ImageTool, v. 2.0, developed by the University of Texas Health Science Center, San Antonio. The kinetics of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lentinula edodes; Shiitake; Substrate; Mycelium growth; Strains.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100017
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Dynamics of the chemical composition and productivity of composts for the cultivation of Agaricus bisporus strains BJM
Andrade,Meire Cristina Nogueira de; Jesus,João Paulo Furlan de; Vieira,Fabrício Rocha; Viana,Sthefany Rodrigues Fernandes; Spoto,Marta Helena Fillet; Minhoni,Marli Teixeira de Almeida.
Two compost formulations based on oat straw (Avena sativa) and brachiaria (Brachiaria sp.) were tested for the cultivation of three Agaricus bisporus strains (ABI-07/06, ABI-05/03, and PB-1). The experimental design was a 2 x 3 factorial scheme (composts x strains) with 6 treatments and 8 repetitions (boxes containing 12 kg of compost). The chemical characterization of the compost (humidity, organic matter, carbon, nitrogen, pH, raw protein, ethereal extract, fibers, ash, cellulose, hemicellulose, and lignin) before and after the cultivation of A. bisporus and the production (basidiomata mass, productivity, and biological efficiency) were evaluated. Data were submitted to variance analysis, and averages were compared by means of the Tukey's test. According...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agaricus bisporus; Productivity; Strains; Compost; Nutritional value.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400016
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Effect of gamma irradiation on the nutritional quality of Agaricus bisporus strains cultivated in different composts Anais da ABC (AABC)
ANDRADE,MEIRE C.N.; JESUS,JOÃO P.F.; VIEIRA,FABRÍCIO R.; VIANA,STHEFANY R.F.; SPOTO,MARTA H.F.; MINHONI,MARLI T.A..
The effect of irradiation doses (0, 125, 250 and 500 Gy) on the nutritional quality of A. bisporus mushrooms (strains ABI-07/06, ABI-05/03 and PB-1) cultivated in composts based on oat straw (Avena sativa) and brachiaria (Brachiaria sp.) was evaluated. The experimental design was 4 x 3 x 2 factorial scheme (irradiation doses x strains x composts), with 24 treatments, consisting of two repetitions each, totaling 48 experimental units (samples of mushrooms). The samples were irradiated in Cobalt-60 irradiator, model Gammacell 220 kGy, with dose rate of 0.740 kGy h–1, according to the treatments proposed. Subsequently, the control (unirradiated) and the other treatments were maintained at 4±1°C and 90% RH in a climatic chamber for carrying out the chemical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agaricus bisporus; Productivity; Strains; Compost; Nutritional value.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200897
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Evaluation of the performance of two strains of Nile tilapia (Oreochromis Niloticus) in mixed raising systems BABT
Neves,Patrícia Ribeiro; Ribeiro,Ricardo Pereira; Vargas,Lauro; Natali,Maria Raquel Marçal; Maehana,Káttia Regina; Marengoni,Nilton Garcia.
The aim of this study was to evaluate the productive performance of two strains of Nile tilapia (Oreochromis niloticus) in mixed raising systems. A total of 3600 fish-larvae species was used, 1800 belonging to Bouaké lineage, and 1800 to Chitralada. The experiment was carried out in three phases; Phase I in an incubator in 18 boxes, in which two treatments (Bouaké and Chitralada) were tested by using nine repetitions; Phases II and III were performed in 18 cement tanks with the same treatments. In phase I, regarding the final weight and gain of weight, Chitralada strain showed the highest final weight values. In phase II, Chitralada showed the highest final weight value when compared with Bouaké, and, considering the gain of weight, Bouaké obtained the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Performance; Strains; Oreochromis niloticus; Reproduction; Nile tilapia.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300012
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Extended serotyping scheme for <i>Vibrio anguillarum</i> with the definition and characterization of seven provisional O-serogroups OMA
Pedersen, K.; Grisez, L.; van Houdt, R.; Tiainen, T.; Ollevier, F.P.; Larsen, J.L..
The present paper summarizes the serotyping scheme of the fish pathogenic bacterium <i>Vibrio anguillarum</i> and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were characterized further by means of LPS profiles, Western blots, and serological reactions. On the basis of the results, it is suggested that the seven new O-serogroups are to be included in the existing serotyping system as serotypes O17-O<sub>2</sub>3. However, the existence of further <i>V. anguillarum</i> strains that were not typable with any of the 23 O-antisera suggested the existence of additional...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diseases; Fish diseases; Pathogenic bacteria; Pathogens; Serological studies; Serotypes; Strains; Vibrio anguillarum.
Ano: 1999 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=9525
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Growth, delayed fluorescence and pigment composition of four Prorocentrum minimum strains growing at two salinities Biol. Res.
BERDEN-ZRIMEC,MAJA; FLANDER-PUTRLE,VESNA; DRINOVEC,LUKA; ZRIMEC,ALEXIS; MONTI,MARINA.
Prorocentrum minimum is a potentially harmful and widely distributed marine dinoflagellate. Several P. minimum strains have already been studied, showing phylogenetical relations of strains isolated from the same geographical regions. Similarity among the strains was further examined on the basis of their physiology. Pigment composition and concentration, as well as delayed fluorescence (DF) decay kinetics and intensity, were measured in four P. minimum strains isolated from the Baltic and Adriatic Seas. The strains were grown at two salinities characteristic of the Baltic (8 PSU) and North Adriatic Seas (32 PSU). Strain differences in DF decay kinetics and growth did not always follow their genetic relations. While two strains showed similarities to the...
Tipo: Journal article Palavras-chave: Adriatic Sea; Baltic Sea; Delayed fluorescence; HPLC; Prorocentrum minimum; Strains.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100003
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Heterogeneity among Strains of Eimeria tenella Isolated from Bangladesh OAK
Karim, M. J.; Begum, N.; Khan, M. S. R..
Palavras-chave: Eimeria; Strains; Heterogeneity; Immunity; Precocious.
Ano: 1994 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/205
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Molecular variants of Grapevine rupestris stem pitting-associated virus infecting grapevines (Vitis spp.) in Brazil Ciência Rural
Fajardo,Thor Vinícius Martins; Kin,Aléxis Cardama; Nickel,Osmar.
ABSTRACT: Grapevine rupestris stem pitting-associated virus (GRSPaV) is one of the most common viruses of grapevine. It is involved in the graft-transmissible disease rupestris stem pitting of the rugose wood complex. The objective of the research was to perform the molecular characterization of the coat protein (CP) gene of sixteen Brazilian GRSPaV isolates aiming to determine the occurrence of molecular variants (strains) of this virus. Nine grapevine samples were evaluated, from which dsRNA was extracted. Nucleotide sequences were generated by Next generation sequencing (NGS). Fifteen complete sequences of the GRSPaV CP gene were obtained and phylogenetically analyzed. Multiple alignments of the sequences showed identities of nucleotides ranging from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: GRSPaV; Foveavirus; Strains; Lineages; Genetic variability.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100153
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Propuesta de una nueva metodología para diferenciar las cepas de Yersinia ruckeri de las de Hafnia alvei OceanDocs
Aguilera, Y.; Martínez, M.T.; Silveira, R..
El objetivo del estudio fue revisar la identificación de dos posibles cepas de Yersinia ruckeri aisladas de diferentes epizootias, diagnosticadas como septicemia bacteriana en 2009 en tilapia cultivada en jaulas, para esclarecer su papel en el cuadro clínico, ya que en los animales afectados no se observaron los signos característicos de la yersiniosis. Se realizó una amplia batería de pruebas bioquímicas mediante técnicas convencionales. La incubación de la motilidad, del Citrato de Simons y del Voges Proskauer, se hizo por duplicado, una réplica se incubó a 25 ºC y la otra, a 37 ºC, lo que permitió identificar a las cepas como Hafnia alvei, que no es un patógeno reconocido de los organismos acuáticos. Se propone un esquema para diferenciar las cepas de...
Tipo: Journal Contribution Palavras-chave: Strains; Biochemical analysis.
Ano: 2011 URL: http://hdl.handle.net/1834/4144
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Xylonic acid: production of xylonic acid by yeast. Infoteca-e
ALMEIDA, J. R. M. de.
Recombinant yeast strains of Komagataella phaffii (Pichia pastoris) and Saccharomyces cerevisiae producers of xylonic acid from xylose in sugarcane biomass hydrolysate.
Tipo: Folders Palavras-chave: Xylonic acid; Fermentation; Strains; Yeasts.
Ano: 2021 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1133059
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Yield of four Agaricus bisporus strains in three compost formulations and chemical composition analyses of the mushrooms BJM
Andrade,Meire Cristina Nogueira de; Zied,Diego Cunha; Minhoni,Marli Teixeira de Almeida; Kopytowski Filho,João.
Three compost formulations, consisting of two varieties of Cynodom dactylon (L.) Pers. (Coast-cross and Tyfton) and oat (Avena sativa) straw were tested for the cultivation of A. bisporus strains ABI-01/01, ABI-04/02, ABI-05/03, and ABI-06/04. A completely randomized experimental design in a factorial scheme was adopted, with 12 treatments (4 A. bisporus strains × 3 types of compost) and 8 replicates. Each experimental unit corresponded to one box containing 12 12.5 kg fresh wet compost. The data were submitted to analysis of variance and the means were compared by Tukey test. According to the results, productivity of mushrooms was influenced by strain and/or compost type. It was also verified that crude protein, ash, and crude fiber contents in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agaricus bisporus; Strains; Straw; Nutritional composition; Productivity.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300034
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